Review




Structured Review

Addgene inc panap
Panap, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/panap+plasmid/pANAP+(Plasmid+%2348696)/pm41290003-347-0-17
Average 93 stars, based on 26 article reviews
panap - by Bioz Stars, 2026-09
93/100 stars

Images

Related Articles

Plasmid Preparation:

Article Title: An Allosteric Model for Electromechanical Coupling in Cardiac CNBD Channels
Article Snippet: Verification of the introduced stop codons within the mutant plasmid was performed through DNA sequencing provided by Genewiz (Azenta Life Sciences, Burlington, MA). .. The incorporation of L-Anap was conducted with the pANAP plasmid (Addgene plasmid #48696), the channel construct with the specific site mutated to amber stop codon , and with 20 μM L-Anap methyl ester (AsisChem, Waltham, MA) in the cell culture. .. To enhance nonsense suppression, a human dominant-negative release factor eRF1 (E55D) (VectorBuilder, Chicago, IL) was co-transfected ( ).

Article Title: A lipid site shapes the agonist response of a pentameric ligand-gated ion channel
Article Snippet: Light was focused onto the dark pole of the oocyte using a Plan Fluor ×40 objective lens (Nikon Instruments), and fluorescence detection was achieved with an H7360–03 photomultiplier (Hamamatsu Photonics) coupled to a PMT400R photomultiplier subsystem (Ionoptix). .. ANAP-labeled ELIC was produced with the pANAP plasmid from Addgene (plasmid No. 48696). .. ELIC macroscopic currents were elicited by the application of 30 mM GABA with a BioLogic fast perfusion system RSC-200 and recorded from several independent experiments ( n = 4 to 26 repeats) with a patch clamp amplifier Axopatch 200 B. ELIC currents were sampled at 40 kHz with an analog filter set to 10 kHz.

Article Title: Nonsubstrate PI(4,5)P 2 interacts with the interdomain linker to control electrochemical coupling in voltage-sensing phosphatase (VSP).
Article Snippet: .. The pAnap plasmid was obtained from Addgene (Addgene ID: 48696) (49). ..

Article Title: Activation-pathway transitions in human voltage-gated proton channels revealed by a non-canonical fluorescent amino acid
Article Snippet: The HEK cells used in this study were obtained from American Type Culture Collection (Cat. # CRL-1573) and were found free of mycoplasma infection (Sigma-Aldrich mycoplasma detection kit). .. These cells were cotransfected with 0.1–1 μg of mutant hH V 1-TAG plasmid and 0.7 μg of pAnap plasmid (a gift from Dr. Sharona Gordon, University of Washington, Seattle, WA, USA; RRID: Addgene_48696 ) using the transfection reagent JetPei (Polyplus-transfection). ..

Article Title: Electromechanical coupling mechanism for activation and inactivation of an HCN channel.
Article Snippet: L-Anap (free-acid form, AsisChem, Waltham, MA) was made as a 1 mM stock in water at a high pH by adding NaOH as previously published15. .. The pANAP plasmid (purchased from Addgene, Cambridge, MA) contained the orthogonal tRNA/aminoacyl-tRNA synthetase specific to L-Anap57. .. Inside-out configuration of the patch-clamp technique was implemented with an EPC-10 (HEKA Elektronik, Germany) or Axopatch 200B (Axon Instruments, Union City, CA) patch-clamp amplifier and PATCHMASTER software (HEKA Elektronik)15.

Article Title: Electromechanical coupling mechanism for activation and inactivation of an HCN channel
Article Snippet: The animal-use protocols were consistent with the recommendations of the American Veterinary Medical Association and were approved by the Institutional Animal Care and Use Committee (IACUC) of the University of Washington. l -Anap (free-acid form, AsisChem, Waltham, MA) was made as a 1 mM stock in water at a high pH by adding NaOH as previously published . .. The pANAP plasmid (purchased from Addgene, Cambridge, MA) contained the orthogonal tRNA/aminoacyl-tRNA synthetase specific to L-Anap . .. Inside-out configuration of the patch-clamp technique was implemented with an EPC-10 (HEKA Elektronik, Germany) or Axopatch 200B (Axon Instruments, Union City, CA) patch-clamp amplifier and PATCHMASTER software (HEKA Elektronik) .

Article Title: Voltage-clamp fluorometry analysis of structural rearrangements of ATP-gated channel P2X2 upon hyperpolarization
Article Snippet: Strain, strain background ( Escherichia coli ) , TG1 , Clontech , , . .. Recombinant DNA reagent , pAnap (plasmid) , Addgene , Plasmid #48696 , cDNA encoding the tRNA synthetase/Anap-CUA. .. Commercial assay or kit , QuikChange II site-directed mutagenesis , Agilent Technologies , 200524 , .

Construct:

Article Title: An Allosteric Model for Electromechanical Coupling in Cardiac CNBD Channels
Article Snippet: Verification of the introduced stop codons within the mutant plasmid was performed through DNA sequencing provided by Genewiz (Azenta Life Sciences, Burlington, MA). .. The incorporation of L-Anap was conducted with the pANAP plasmid (Addgene plasmid #48696), the channel construct with the specific site mutated to amber stop codon , and with 20 μM L-Anap methyl ester (AsisChem, Waltham, MA) in the cell culture. .. To enhance nonsense suppression, a human dominant-negative release factor eRF1 (E55D) (VectorBuilder, Chicago, IL) was co-transfected ( ).

Cell Culture:

Article Title: An Allosteric Model for Electromechanical Coupling in Cardiac CNBD Channels
Article Snippet: Verification of the introduced stop codons within the mutant plasmid was performed through DNA sequencing provided by Genewiz (Azenta Life Sciences, Burlington, MA). .. The incorporation of L-Anap was conducted with the pANAP plasmid (Addgene plasmid #48696), the channel construct with the specific site mutated to amber stop codon , and with 20 μM L-Anap methyl ester (AsisChem, Waltham, MA) in the cell culture. .. To enhance nonsense suppression, a human dominant-negative release factor eRF1 (E55D) (VectorBuilder, Chicago, IL) was co-transfected ( ).

Produced:

Article Title: A lipid site shapes the agonist response of a pentameric ligand-gated ion channel
Article Snippet: Light was focused onto the dark pole of the oocyte using a Plan Fluor ×40 objective lens (Nikon Instruments), and fluorescence detection was achieved with an H7360–03 photomultiplier (Hamamatsu Photonics) coupled to a PMT400R photomultiplier subsystem (Ionoptix). .. ANAP-labeled ELIC was produced with the pANAP plasmid from Addgene (plasmid No. 48696). .. ELIC macroscopic currents were elicited by the application of 30 mM GABA with a BioLogic fast perfusion system RSC-200 and recorded from several independent experiments ( n = 4 to 26 repeats) with a patch clamp amplifier Axopatch 200 B. ELIC currents were sampled at 40 kHz with an analog filter set to 10 kHz.

Mutagenesis:

Article Title: Activation-pathway transitions in human voltage-gated proton channels revealed by a non-canonical fluorescent amino acid
Article Snippet: The HEK cells used in this study were obtained from American Type Culture Collection (Cat. # CRL-1573) and were found free of mycoplasma infection (Sigma-Aldrich mycoplasma detection kit). .. These cells were cotransfected with 0.1–1 μg of mutant hH V 1-TAG plasmid and 0.7 μg of pAnap plasmid (a gift from Dr. Sharona Gordon, University of Washington, Seattle, WA, USA; RRID: Addgene_48696 ) using the transfection reagent JetPei (Polyplus-transfection). ..

Transfection:

Article Title: Activation-pathway transitions in human voltage-gated proton channels revealed by a non-canonical fluorescent amino acid
Article Snippet: The HEK cells used in this study were obtained from American Type Culture Collection (Cat. # CRL-1573) and were found free of mycoplasma infection (Sigma-Aldrich mycoplasma detection kit). .. These cells were cotransfected with 0.1–1 μg of mutant hH V 1-TAG plasmid and 0.7 μg of pAnap plasmid (a gift from Dr. Sharona Gordon, University of Washington, Seattle, WA, USA; RRID: Addgene_48696 ) using the transfection reagent JetPei (Polyplus-transfection). ..

Recombinant:

Article Title: Voltage-clamp fluorometry analysis of structural rearrangements of ATP-gated channel P2X2 upon hyperpolarization
Article Snippet: Strain, strain background ( Escherichia coli ) , TG1 , Clontech , , . .. Recombinant DNA reagent , pAnap (plasmid) , Addgene , Plasmid #48696 , cDNA encoding the tRNA synthetase/Anap-CUA. .. Commercial assay or kit , QuikChange II site-directed mutagenesis , Agilent Technologies , 200524 , .



Similar Products

93
Addgene inc panap
Panap, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/panap+plasmid/pANAP+(Plasmid+%2348696)/pm41290003-347-0-17
Average 93 stars, based on 1 article reviews
panap - by Bioz Stars, 2026-09
93/100 stars
  Buy from Supplier

93
Addgene inc peter schultz
Peter Schultz, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/panap+plasmid/pANAP+(Plasmid+%2348696)/pmc12652999-255-52-54
Average 93 stars, based on 1 article reviews
peter schultz - by Bioz Stars, 2026-09
93/100 stars
  Buy from Supplier

93
Addgene inc panap vector
Panap Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/panap+plasmid/pANAP+(Plasmid+%2348696)/pm41116071-301-8-13
Average 93 stars, based on 1 article reviews
panap vector - by Bioz Stars, 2026-09
93/100 stars
  Buy from Supplier

93
Addgene inc panap plasmid
Panap Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/panap+plasmid/pANAP+(Plasmid+%2348696)/bio_rxiv__2025__07__28__666606-121-8-10
Average 93 stars, based on 1 article reviews
panap plasmid - by Bioz Stars, 2026-09
93/100 stars
  Buy from Supplier

93
Addgene inc sert panap
Sert Panap, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/panap+plasmid/pANAP+(Plasmid+%2348696)/pm39463388-266-5-8
Average 93 stars, based on 1 article reviews
sert panap - by Bioz Stars, 2026-09
93/100 stars
  Buy from Supplier

Image Search Results